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A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with <t>Periodic</t> <t>acid–Schiff</t> (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.
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A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with <t>Periodic</t> <t>acid–Schiff</t> (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.
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A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with <t>Periodic</t> <t>acid–Schiff</t> (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.
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A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with <t>Periodic</t> <t>acid–Schiff</t> (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.
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Image Search Results


A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with Periodic acid–Schiff (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.

Journal: EMBO Molecular Medicine

Article Title: Nanog maintains stemness of Lkb1 ‐deficient lung adenocarcinoma and prevents gastric differentiation

doi: 10.15252/emmm.202012627

Figure Lengend Snippet: A, B Human (A) and KLN murine (B) lung sections were stained with H&E or with Periodic acid–Schiff (PAS) and Alcian blue (AB). Scale bar, 50 μm. C Statistical analysis of mucinous tumor numbers (no.) in KL ( n = 7) or KLN ( n = 7) mice. Significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. Results were shown as mean. ** P = 0.0066. D Representative pictures of lung tumors at 4 weeks and 8 weeks post‐Ad‐Cre infection in KLN mice. Scale bar, 50 μm. E Statistical analysis of IMA ratio at 4 and 8 weeks post‐Ad‐Cre treatment ( n = 5 per group). Results were shown as mean ± SEM. F Statistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC ( n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one‐way ANOVA with Dunnett’s multiple comparisons test. * P = 0.0215. G qPCR analysis of stomach‐restricted gene set between KL ADC and KLN mucinous tumor sections ( n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two‐tailed unpaired Student’s t ‐test with Welch’s correction. * P = 0.0260 ( Hnf4α ), * P = 0.0275 ( Vsig1 ), * P = 0.0122 ( Gkn3 ), * P = 0.0324 ( Ctse ), ** P = 0.0084 ( Onecut2 ). H Representative histological and IHC staining for HNF4 α, GKN1, NKX2‐1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 μm.

Article Snippet: For Alcian Blue and PAS staining, tumor sections were stained with 1% Alcian Blue pH 2.5 and Periodic acid–Schiff reagent (Polyscientific Company) according to the manufacturer’s instructions.

Techniques: Staining, Two Tailed Test, Infection, Whisker Assay, Immunohistochemistry, Derivative Assay